Napoli are forced to reshuffle with Chucky Lozano as a centre-forward flanked by Matteo Politano and Lorenzo Insigne against giant-killers Spezia.
It kicks off at 20.00 GMT at the Stadio Diego Armando Maradona and the winners go on to face Atalanta in the two-legged semi-final.
However, tonight’s tie will be decided after 90 minutes, extra time or even penalties.
Napoli are forced to reshuffle with Chucky Lozano as a centre-forward flanked by Matteo Politano and Lorenzo Insigne against giant-killers Spezia.
It kicks off at 20.00 GMT at the Stadio Diego Armando Maradona and the winners go on to face Atalanta in the two-legged semi-final.
However, tonight’s tie will be decided after 90 minutes, extra time or even penalties.
You can follow all the build-up and action as it happens on the LIVEBLOG.
The Partenopei are feeling the tension after a series of bad results, including the Italian Supercup defeat to Juventus and fumbling a lead to fall 3-1 at Hellas Verona in Serie A on Sunday.
There were even reports Gennaro Gattuso would be fired, so yesterday President Aurelio De Laurentiis released a statement confirming his ‘full confidence’ in the coach.
Victor Osimhen and Dries Mertens are back, but nowhere near fully fit, with Andrea Petagna also carrying a knock.
Therefore, Lozano is placed in a centre-forward role with support from Politano, Insigne and Piotr Zielinski.
Spezia are in their debut Serie A season, but have already impressed with strong results and performances, then knocked Roma out in the Coppa Italia Round of 16.
Riccardo Saponara scored two goals in that 4-2 win, but he is out injured with M’Bala Nzola, Roberto Piccoli, Tommaso Pobega and Federico Mattiello.
Goalkeeper Ivan Provedel is again rested, so Titas Krapikas takes the gloves in the Coppa.
Andrij Galabinov is the only real remaining striker, flanked by Daniele Verde and Emanuel Gyasi.
Napoli: Ospina; Hysaj, Manolas, Koulibaly, Mario Rui; Elmas, Demme, Zielinski; Politano, Lozano, Insigne
Spezia: Krapikas; Vignali, Ismajli, Terzi, Dell’Orco; Estevez, Ricci, Acampora; Gyasi, Galabinov, Verde