It’s a head-to-head for Champions League places, as Atalanta look to Luis Muriel and Jeremie Boga, while Juventus start Dusan Vlahovic, Paulo Dybala and Alvaro Morata together.
It kicks off at the Gewiss Stadium in Bergamo at 19.45 GMT.
You can follow all the build-up and action as it happens on the LIVEBLOG.
Liveblog: Serie A Wk25 inc Milan-Sampdoria, Sassuolo-Roma, Atalanta-Juventus
The Old Lady leapfrogged La Dea in the standings last week, but Atalanta still have a game in hand, so that could change.
This has been perhaps the toughest time since Gian Piero Gasperini took over in Bergamo, especially on home turf, as they crashed out of the Coppa Italia to 10-man Fiorentina midweek.
Duvan Zapata is out for at least another couple of months, with Josip Ilicic still dealing with mental health issues.
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Aleksei Miranchuk and Jose Luis Palomino are also injured, with goalkeeper Juan Musso suspended.
January signing Boga and Teun Koopmeiners support Luis Muriel upfront.
Juventus, on the other hand, are feeling exceptionally confident after transforming the squad in January.
Dusan Vlahovic and Denis Zakaria both scored on their debuts, with the Serbian also creating the own goal that secured their Coppa Italia win against Sassuolo.
Giorgio Chiellini, Federico Bernardeschi and Federico Chiesa are out injured, but Leonardo Bonucci returns in defence.
Max Allegri opts for the all-attack trident of Vlahovic with Dybala and Morata, but the change is in midfield, with Zakaria sent to the bench.
Atalanta: Sportiello; Toloi, Demiral, Djimsiti; Hateboer, De Roon, Freuler, Zappacosta; Koopmeiners; Muriel, Boga
Juventus: Szczesny; Danilo, Bonucci, De Ligt, De Sciglio; McKennie, Locatelli, Rabiot; Dybala, Vlahovic, Morata