Mario Mandzukic and Paulo Dybala face Edin Dzeko and Mohamed Salah, but Roma and Juventus make surprise changes.
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These clubs battled for the Scudetto in recent years and started out as favourites for the title once again.
They both had a shaky opening round, as the Giallorossi were held 1-1 at Verona and the Bianconeri beaten at home by Udinese.
Mario Mandzukic and Paulo Dybala face Edin Dzeko and Mohamed Salah, but Roma and Juventus make surprise changes.
It kicks off at 17.00 UK time (16.00 GMT), join in our LIVEBLOG.
These clubs battled for the Scudetto in recent years and started out as favourites for the title once again.
They both had a shaky opening round, as the Giallorossi were held 1-1 at Verona and the Bianconeri beaten at home by Udinese.
Juve have never lost the opening two games in a season, so they could make history at the Stadio Olimpico.
Alvaro Morata is fit again, but is on the bench, as Paulo Dybala steps in to partner Mandzukic in attack.
Simone Padoin again takes the role in front of the defence, as Claudio Marchisio, Sami Khedira and Kwadwo Asamoah are injured.
Juan Guillermo Cuadrado and Alex Sandro are on the bench after arriving over the last week, but Martin Caceres is picked rather than Andrea Barzagli in a back three.
Roma said Lucas Digne would not debut from the start just days after moving from Paris Saint-Germain, but the Frenchman is thrown straight into the mix at left-back.
Alessandro Florenzi is again chosen ahead of not quite fit Douglas Maicon on the right, but above all Daniele De Rossi is moved into centre-back.
Iago Falque takes over from Gervinho in the trident attack.
Dzeko faces Juventus for the first time in his career, while Salah famously bagged a brace against the Bianconeri with Fiorentina last term.
Francesco Totti is again left to watch from the sidelines, while Kevin Strootman and Antonio Rudiger are injured.
Roma: Szscesny; Florenzi, Manolas, De Rossi, Digne; Pjanic, Keita, Nainggolan; Salah, Dzeko, Falque
Roma bench: De Sanctis, Castan, Capradossi, Gyomber, Anocic, Paredes, Ucan, Totti, Ibarbo, Gervinho, Iturbe, Ljajic
Juventus: Buffon; Caceres, Bonucci, Chiellini; Lichsteiner, Sturaro, Padoin, Pogba, Evra; Dybala, Mandzukic