Fiorentina rely on Federico Bernardeschi and Mauro Zarate against Tottenham in the Europa League Round of 32.
It kicks off at the Stadio Artemio Franchi at 18.00 GMT, follow the action from this game, Villarreal-Napoli and Galatasaray-Lazio on our LIVEBLOG.
The Viola climbed back into third place in Serie A at the weekend thanks to a last-gasp 2-1 victory over Inter.
Fiorentina rely on Federico Bernardeschi and Mauro Zarate against Tottenham in the Europa League Round of 32.
It kicks off at the Stadio Artemio Franchi at 18.00 GMT, follow the action from this game, Villarreal-Napoli and Galatasaray-Lazio on our LIVEBLOG.
The Viola climbed back into third place in Serie A at the weekend thanks to a last-gasp 2-1 victory over Inter.
Coach Paulo Sousa rotates his squad and January signing Zarate starts because he will sit out a three-round Serie A ban.
With Josip Ilicic, Jakub Blaszczykowski and Bernardeschi – who has Italy Coach Antonio Conte in the stands to watch him today.
Nenad Tomovic steps in for the suspended Facundo Roncaglia.
Spurs are mounting the first genuine Premier League title challenge for decades and beat Manchester City on Sunday, but goalkeeper and captain Hugo Lloris is ruled out by a shoulder injury.
Erik Lamela is a familiar figure from his time at Roma and this term scored five goals in five Europa League games, but he is surprisingly not even on the bench.
Instead 19-year-old Dele Alli is one to watch.
Nacer Chadli gets the nod upfront rather than Harry Kane in Florence, while Clinton Njie is injured.
These clubs met in the Europa League last season and Fiorentina went through 3-1 on aggregate, having drawn 1-1 in London and celebrated a 2-0 victory on home turf.
No English side has ever beaten the Viola at the Stadio Franchi or even scored here with one draw and four defeats.
Sousa’s men have stuttered in the Europa League so far, losing two home fixtures on the bounce before a 1-0 win over Belenenses.
On the other hand, Spurs won only one of their last five European away games, 1-0 at Qarabag.
Fiorentina: Tatarusanu; Tomovic, Gonzalo Rodriguez, Astori, Marcos Alonso; Tino Costa, Borja Valero; Blaszczykowski, Ilicic, Bernardeschi; Zarate
Fiorentina bench: Sepe, Pasqual, Badelj, Vecino, Mati Fernandez, Kalinic, Babacar
Tottenham: Vorm; Trippier, Alderweireld, Wimmer, Davies; Carroll, Mason; Eriksen, Alli, Son; Chadli
Tottenham bench: McGee, Rose, Walker, Dembele, Dier, Onomah, Kane
Ref: Zwayer (GER)