Juventus start Federico Chiesa against his old club with Moise Kean, while Lucas Beltran and Nico Gonzalez lead the Fiorentina attack.
It kicks off at the Stadio Artemio Franchi at 19.45 GMT.
There were fears this fixture would not go ahead after flash floods killed seven in the region, but the rain has now stopped. Nonetheless, Fiorentina ultras are boycotting the match and leave the stands empty, instead working to help clear the mud.
This has always been a bitter grudge match between rival clubs and the tension has only escalated in recent years with the transfers of Chiesa and Dusan Vlahovic to Turin.
Only the Italian starts here this evening, the first time he has faced his former teammates, but Vlahovic is not 100 per cent fit and replaced by Kean.
Timothy Weah, Danilo, Alex Sandro and Mattia De Sciglio are injured, with Paul Pogba and Nicolò Fagioli suspended over off the field issues.
Fiorentina had been ready to break into the top four until back-to-back defeats to Empoli and Lazio, the latter decided by a last-gasp Ciro Immobile penalty.
Michael Kayode, Dodo and Gaetano Castrovilli are on the treatment table, so Alessandro Parisi has to switch over as a makeshift right-back.
Arthur Melo is on loan from Juventus with option to buy and eager to make an impact, but Giacomo Bonaventura is surprisingly benched.
Lucas Beltran leads the attack with Nico Gonzalez, Antonin Barak and Christian Kouame.
Fiorentina: Terracciano; Parisi, Ranieri, Martinez Quarta, Biraghi; Arthur, Mandragora; Gonzalez, Barak, Kouame; Beltran
Juventus: Szczesny; Gatti, Bremer, Rugani; McKennie, Miretti, Locatelli, Rabiot, Kostic; Kean, Chiesa
Player statistic
| 10' |  |
Fabio Miretti (Assist: Filip Kostic) | ||
| 43' |  |
Adrien Rabiot |
| 48' | |
Moise Kean | ||
| 58' | |
Federico Gatti | ||
| Luca Ranieri | |
83' |
Match statistic
