Leonardo Spinazzola, Antonio Mirante, Pedro Rodriguez and Riccardo Calafiori are out of the Roma squad to face Sampdoria tomorrow.
It kicks off on Sunday at 14.00 GMT.
Both Pedro and Calafiori have been diagnosed with a thigh strain and will be evaluated on a daily basis.
Defender Federico Fazio has a left knee issue and is also out of the squad.
Goalkeeper Mirante has been struggling over the last few weeks with fitness issues and his absence means Pau Lopez takes over in goal.
Leonardo Spinazzola, Antonio Mirante, Pedro Rodriguez and Riccardo Calafiori are out of the Roma squad to face Sampdoria tomorrow.
It kicks off on Sunday at 14.00 GMT.
Both Pedro and Calafiori have been diagnosed with a thigh strain and will be evaluated on a daily basis.
Defender Federico Fazio has a left knee issue and is also out of the squad.
Goalkeeper Mirante has been struggling over the last few weeks with fitness issues and his absence means Pau Lopez takes over in goal.
Spinazzola has been on sparkling form lately, but he sustained a thigh strain on December 20.
Other long-term absentees are Javier Pastore and Nicolò Zaniolo.
Roma squad for Sampdoria: Farelli, Pau Lopez, Boer, Karsdorp, Ibanez, Juan Jesus, Smalling, Santon, Mancini, Kumbulla, Peres, Cristante, Pellegrini, Villar, Veretout, Diawara, Darboe, Podgoreanu, Mkhitaryan, Dzeko, Mayoral, Perez, Providence