Atalanta are forced to reshuffle yet again in defence amid an injury crisis, looking to Josip Ilicic and Duvan Zapata upfront, as Lazio have Pedro and Felipe Anderson supporting Ciro Immobile.
It kicks off at the Gewiss Stadium in Bergamo at 14.00 UK time (13.00 GMT).
Gian Piero Gasperini is continuing to deal with an injury crisis that has ruled out Berat Djimsiti, Rafael Toloi, Robin Gosens, Matteo Pessina and Hans Hateboer.
Jose Luis Palomino is suspended, so Merih Demiral must rush his return from a muscular problem, flanked by teenager Matteo Lovato and makeshift defender Marten de Roon.
Mario Pasalic and Ilicic are chosen upfront to support Duvan Zapata, but there’s no room to rotate ahead of the Champions League showdown with Manchester United.
Lazio are incredibly inconsistent, especially on their travels, after heavy defeats to Verona and Bologna.
The team was in a training retreat after that 4-1 loss at the Stadio Bentegodi and came out of it by beating Fiorentina 1-0 in the midweek round.
Mattia Zaccagni is on the treatment table, while Luis Alberto is started for the second game in a row with Danilo Cataldi in the middle rather than Lucas Leiva.
Felipe Anderson and Pedro support striker Ciro Immobile, with Sergej Milinkovic-Savic in a free role.
Atalanta: Musso; De Roon, Demiral, Lovato; Zappacosta, Koopmeiners, Freuler, Maehle; Pasalic; Ilicic, Duvan Zapata
Lazio: Reina; Marusic, Luiz Felipe, Acerbi, Hysaj; Milinkovic-Savic, Cataldi, Luis Alberto; Felipe Anderson, Immobile, Pedro
Strakosha uno di noi! Forza Lazio!