Atalanta start Papu Gomez, Duvan Zapata and Matteo Pessina in a more advanced role, despite recent tension, as they must avoid defeat to Ajax in Amsterdam.
It kicks off at the Johan Cruijff Arena at 17.55 GMT.
You can follow all the build-up and action as it happens from that game and Inter-Shakhtar Donetsk on the LIVEBLOG.
Atalanta start Papu Gomez, Duvan Zapata and Matteo Pessina in a more advanced role, despite recent tension, as they must avoid defeat to Ajax in Amsterdam.
It kicks off at the Johan Cruijff Arena at 17.55 GMT.
La Dea are on the verge of securing their place in the Champions League Last 16 for the second year in a row, not to mention the second time in their history that they have been in this tournament.
There was genuine tension building up to this trip, with Gomez and Josip Ilicic dropped following reports of a furious locker room row with coach Gian Piero Gasperini during the 1-1 draw with Midtjylland.
They were left out of Sunday’s Serie A visit to Udinese, but that game was called off due to a waterlogged pitch, so at least the players are well rested.
Both are back, even if Papu is the only one starting, with Pessina chosen to support Duvan Zapata in the absence of injured Mario Pasalic.
Robin Gosens returns after his COVID test proved to be a false positive and Pierluigi Gollini is also available following a knock.
Mattia Caldara, Pasalic and Aleksei Miranchuk are still out of action.
While Atalanta can qualify with a point, Ajax need the victory to go through, and will throw everything at the Italians.
Daley Blind, Mohammed Kudus, David Neres and Lassina Traore are out injured, while Quincy Promes is on the bench.
Antony, Dusan Tadic and Brian Brobbey form the trident attack, but talented teenager Ryan Gravenberch is already attracting attention from Europe’s top clubs.
This is Brobbey's first senior start in the Ajax jersey, he is yet another Mino Raiola player, and has been called the 'new Romelu Lukaku.'
Ajax: Onana; Mazraoui, Schuurs, Martinez, Tagliafico; Klaassen, Labyad, Gravenberch; Antony, Brobbey, Tadic
Ajax bench: Stekelenberg, Kotarski, J Timber, Alvarez, Klaiber, Huntelaar, Promes, Rensch, Ekkelenkamp
Atalanta: Gollini; Toloi, Romero, Djimsiti; Hateboer, De Roon, Freuler; Gosens; Gomez, Pessina; Duvan Zapata
Atalanta bench: Rossi, Sportiello, Palomino, Lammers, Muriel, Malinovskyi, Piccini, Mojica, Depaoli, Ruggeri, Panada, Ilicic
Ref: Del Cerro Grande (ESP)