Napoli give Stanislav Lobotka and Diego Demme their first starts, while Coppa Italia holders Lazio use a full-strength team in the quarter-final.
It kicks off at the Stadio San Paolo at 19.45 GMT.
This is a one-off match, so the winner will be decided after 90 minutes, extra time or even penalties.
Gennaro Gattuso is in dire straits after losing four of his first five Serie A games in charge, while he got through the Coppa Italia Round of 16 thanks to a couple of Lorenzo Insigne penalties against Serie B side Perugia.
Napoli give Stanislav Lobotka and Diego Demme their first starts, while Coppa Italia holders Lazio use a full-strength team in the quarter-final.
It kicks off at the Stadio San Paolo at 19.45 GMT.
This is a one-off match, so the winner will be decided after 90 minutes, extra time or even penalties.
Gennaro Gattuso is in dire straits after losing four of his first five Serie A games in charge, while he got through the Coppa Italia Round of 16 thanks to a couple of Lorenzo Insigne penalties against Serie B side Perugia.
The coach rings the changes to get them back on track, with January signing Lobotka making his absolute debut and Demme given his first start after two substitute appearances.
David Ospina is again preferred to Alex Meret in goal, while Kalidou Koulibaly, Nikola Maksimovic, Faouzi Ghoulam, Kevin Malcuit, Allan and Dries Mertens are out injured.
Lazio use practically a full-strength squad, as winning 11 Serie A games in a row is not enough for coach Simone Inzaghi, who in the last seven months has also won the Coppa Italia and the Italian Super Cup.
Ciro Immobile faces his hometown club and is partnered by Felipe Caicedo, as Joaquin Correa is only fit for the bench.
The Biancocelesti use their star names Sergej Milinkovic-Savic and Lucas Leiva, but Luis Alberto, Danilo Cataldi, Adam Marusic and Jordan Lukaku are out injured.
The winners of this fixture will go on to face Fiorentina or Inter in the two-legged semi-finals.
Napoli: Ospina; Hysaj, Manolas, Di Lorenzo, Mario Rui; Lobotka, Demme, Zielinski; Callejon, Milik, Insigne
Napoli bench: Meret, Karnezis, Luperto, Tonelli, Elmas, Ruiz, Lozano, Leandrinho, Llorente
Lazio: Strakosha; Luiz Felipe, Acerbi, Radu; Lazzari, Milinkovic, Leiva, Parolo, Lulic; Immobile, Caicedo
Lazio bench: Proto, Guerrieri, Bastos, Patric, Vavro, D. Anderson, Minala, Berisha, A. Anderson, Jony, Correa, Adekanye
Ref: Massa
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