Empoli and Bologna meet at the Stadio Castellani, with Riccardo Orsolini and Francesco Caputo expected to start.
It kicks off at 19:45 GMT+1, the same kick-off time as Udinese-Napoli.
Koni De Winter is the only player injured for the Tuscans. Sebastian Walukiewicz and Sebastiano Luperto are in contention to start at centre-back alongside Ardian Ismajli, while Jean-Louis Akpa Akpro is favourite over Nicolas Haas in the middle of the park.
Empoli are 13 points below Bologna in the Serie A table but have remarkable records against the Rossoblu in Serie A.
They are unbeaten in nine of the last 10 Serie A games with Bologna and won the reverse fixture. The Tuscans also won the last three home meetings with the Rossoblu.
However, no team in Serie A has collected fewer points than the Tuscans since the start of February, only six in 12 matches.
Bologna boss Thiago Motta is short of options in attack without the injured Marko Arnautovic, Nicola Sansone and Roberto Soriano, while Giorgos Kyriakopoulos is serving a one-match ban.
Orsolini and Lewis Ferguson are expected to start alongside Musa Barrow in attack with Michel Aebischer initially on the bench.
Scorer of nine goals so far this season, Orsolini could reach double figures for goals for the first time in his career.
Empoli-Bologna: probable line-ups
Empoli (4-3-1-2): Vicario; Ebuhei, Ismajli, Luperto, Parisi; Haas, Marin, Bandinelli; Caputo, Cambiaghi.
Bologna (4-3-3): Skorupski; Posch, Soumaoro, Lucumi, Cambiaso; Moro, Schouten, Dominguez; Orsolini, Barrow, Ferguson.