Danilo has not recovered from an ankle problem and Max Allegri will decide whether to unveil a new tactical system against Frosinone.
It kicks off at the Allianz Juventus Stadium on Sunday at 11.30 GMT.
Max Allegri named his squad for this match, but it does not include Danilo, Moise Kean, Mattia Perin or Mattia De Sciglio, although Alex Sandro is present.
Captain Danilo sustained an ankle injury during the 2-2 draw with Verona and should be replaced in defence by Daniele Rugani.
Although he had tested out the 4-3-3 formation in training this week, Allegri does look set to stick with the 3-5-2 tactics, using Dusan Vlahovic and Federico Chiesa upfront.
Some changes could see January signing Carlos Alcaraz get his first start in midfield rather than Fabio Miretti.
Frosinone are missing Giuseppe Caso, Anthony Oyono, Marvin Cuni, Kevin Bonifazi, Mateus Lusuardi, Sergio Kalaj and Riccardo Marchizza.
The positive news is the return to the squad of Nadir Zortea, Luca Garritano and Fares Khedjemis.
January signing Isak Vural gets his first call-up, but all eyes are on Juventus-owned Enzo Barrenechea, Kaio Jorge and Matias Soulè.
Probable line-ups
Juventus (3-5-2): Szczesny; Gatti, Bremer, Rugani; McKennie, Alcaraz, Locatelli, Rabiot, Cambiaso; Vlahovic, Chiesa
Frosinone (4-3-3): Turati; Lirola, Okoli, Romagnoli, Valeri; Gelli, Barrenechea, Mazzitelli; Soulè, Kaio Jorge, Harroui
La lista dei convocati per il match in programma domani 📜⚪️⚫️@zondacrypto #JuveFrosinone pic.twitter.com/dlaSD9hn3K
— JuventusFC (@juventusfc) February 24, 2024