Francesco Totti, Gervinho and Juan Iturbe start as Roma host Bayern Munich in the Champions League, but Douglas Maicon is injured.
Francesco Totti, Gervinho and Juan Iturbe start as Roma host Bayern Munich in the Champions League, but Douglas Maicon is injured.
It kicks off at the Stadio Olimpico at 19.45 UK time (18.45 GMT) and is a potentially decisive game for progress into the next round.
There was another huge boost for their chances of qualification when Manchester City fumbled a 2-0 lead to draw 2-2 away to CSKA Moscow.
City now have just two points from three games, while Bayern have six from two matches and Roma four points.
Bayern Munich arrived 30 minutes late to the Olimpico due to traffic.
The Giallorossi have four points after crushing CSKA Moscow 5-1 and earning a 1-1 away to Manchester City, while Bayern beat both City and CSKA with 1-0 scorelines.
Gervinho and Iturbe were rested for the 3-0 win over Chievo on Saturday, but return to partner captain Totti in Rudi Garcia’s traditional 4-3-3 formation.
Morgan De Sanctis and Daniele De Rossi have also shaken off knocks, but Douglas Maicon picked up a knee problem just hours before the game and is replaced by Vasilis Torosidis at right-back.
There are still several players on the treatment table, including Seydou Keita, Leandro Castan and Kevin Strootman.
Bayern Munich won their Bundesliga fixture 6-0 on Saturday and are the absolute favourites to top this group.
It is a return to the Olimpico for Guardiola, who played for Roma towards the end of his career.
Missing out are Holger Badstuber, Javi Martinez, Pepe Reina, Bastian Schweinsteiger, Tom Starke and Thiago Alcantara.
Franck Ribery made his comeback this week and is raring to prove his fitness in the second half, while Arjen Robben is always a tricky customer.
German World Cup hero Mario Gotze also starts with Thomas Muller, Robert Lewandowski and Xabi Alonso in a star-studded team.
Expect an extremely frosty reception for Mehdi Benatia, who left Roma in bitter circumstances just two months ago and is surprisingly in the starting XI tonight.
The referee is Swede Jonas Eriksson and Bayern are unbeaten in 11 competitive games.
The Stadio Olimpico is completely sold out with 70,000 spectators present.
Roma: De Sanctis; Torosidis, Manolas, Yanga Mbiwa, Cole; Pjanic, De Rossi, Nainggolan; Gervinho, Totti, Iturbe
Roma bench: Skorupski, Astori, Holebas, Florenzi, Paredes, Destro, Ljajic
Bayern Munich: Neuer; Lahm, Boateng, Benatia, Bernat; Alaba, Xabi Alonso; Muller, Gotze; Robben; Lewandoski
Bayern bench: Dante, Rafinha, Shaqiri, Ribery, Pizarro, Højbjerg