Last year’s Finalists face off again in the quarter-final, as Lazio and Juventus go head-to-head in Rome.
It kicks off at 19.45 GMT and will be decided tonight after 90 minutes, extra time or penalties.
The winner goes through to face Inter in the semi-finals, while the other semi is between Milan and Lega Pro fairy-tale side Alessandria.
Juventus won when these teams last clashed in the 2015 Coppa Italia Final and again in the Italian Super Cup in Shanghai.
Last year’s Finalists face off again in the quarter-final, as Lazio and Juventus go head-to-head in Rome.
It kicks off at 19.45 GMT and will be decided tonight after 90 minutes, extra time or penalties.
The winner goes through to face Inter in the semi-finals, while the other semi is between Milan and Lega Pro fairy-tale side Alessandria.
Juventus won when these teams last clashed in the 2015 Coppa Italia Final and again in the Italian Super Cup in Shanghai.
The Bianconeri have won their last 10 Serie A games and it remains to be seen if they can maintain that stunning form in the Coppa as well.
Sami Khedira, Andrea Barzagli, Patrice Evra, Roberto Pereyra and Mario Lemina are unavailable, while the likes of Paulo Dybala, Mario Mandzukic and Gigi Buffon are rested.
It gives Simone Zaza and Alvaro Morata the chance to shine, but Hernanes, Daniele Rugani and Simone Padoin do not get expected starts, as Max Allegri keeps squad rotation to a minimum.
Lazio have a huge injury list to contend with, including Federico Marchetti, Ricardo Kishna, Santiago Gentiletti, Stefan de Vrij, Dusan Basta and Ravel Morrison.
The Aquile give new defender Milan Bisevac his debut in defence, with Keita Balde Diao and Miroslav Klose completing the attack.
Lazio: Berisha; Konko, Biśevac, Mauricio, Radu; Milinkovic-Savic, Biglia, Lulic; Candreva, Klose, Keita
Lazio bench: Guerrieri, Matosevic, Hoedt, Braafheid, Patric, Parolo, Onazi, Cataldi, Felipe Anderson, Mauri, Matri, Djordjevic
Juventus: Neto; Caceres, Bonucci, Chiellini; Lichtsteiner, Sturaro, Marchisio, Pogba, Alex Sandro; Zaza, Morata
Juventus bench: Buffon, Audero, Rugani, Padoin, Hernanes, Asamoah, Cuadrado, Mandzukic, Dybala