Fiorentina go head-to-head with Roma in a Europa League Round of 16 all-Italian derby, having already met in the Coppa Italia.
This first leg kicks off at 20.05 GMT at the Stadio Artemio Franchi.
It will be the second time these sides have clashed in a knockout competition this season, as Fiorentina won 2-0 at the Olimpico to knock Roma out of the Coppa Italia.
There are five Serie A sides left in the Last 16 of the Europa League, the most representing any nation at this stage since West Germany in the 1979-80 UEFA Cup.
Fiorentina go head-to-head with Roma in a Europa League Round of 16 all-Italian derby, having already met in the Coppa Italia.
This first leg kicks off at 20.05 GMT at the Stadio Artemio Franchi.
It will be the second time these sides have clashed in a knockout competition this season, as Fiorentina won 2-0 at the Olimpico to knock Roma out of the Coppa Italia.
There are five Serie A sides left in the Last 16 of the Europa League, the most representing any nation at this stage since West Germany in the 1979-80 UEFA Cup.
Both teams have numerous injury problems and reason to seek a morale-boosting result here today.
The Viola had been unbeaten in 12 competitive games before their 4-0 hammering by Lazio on Monday evening.
Stefan Savic, Mario Gomez, Khouma Babacar, Federico Bernardeschi, Ciprian Tatarusanu and Giuseppe Rossi are unavailable.
Mohamed Salah had his first bad game since arriving from Chelsea, so wants that momentum back.
Roma fell 11 points behind Serie A leaders Juventus thanks to a remarkable series of stalemates and confidence is very low after Rudi Garcia described their performance against Chievo as “disconcerting.”
Francesco Totti, Seydou Doumbia, Victor Ibarbo, Douglas Maicon are on the treatment table, so there’s a new trident with Alessandro Florenzi, Adem Ljajic and Juan Manuel Iturbe.
The Giallorossi have won only two European games this season, against CSKA Moscow in the Champions League and Feyenoord in the Europa League, split by three draws and three losses.
Fiorentina: Neto; Tomovic, Gonzalo Rodriguez, Basanta, Alonso; Badelj, Pizarro, Borja Valero; Joaquin, Ilicic, Salah
Fiorentina bench: Lezzerini, Richards, Pasqual, Vargas, Aquilani, Mati Fernandez, Babacar
Roma: Skorupski; Torosidis, Manolas, Yanga-Mbiwa, Holebas; Nainggolan, De Rossi, Keita; Florenzi, Ljajic, Iturbe
Roma bench: De Sanctis, Cole, Astori, Pjanic, Gervinho, Verde, Doumbia