Italy could make four or five changes to the starting XI for the final Group A game against Wales, potentially including Marco Verratti’s Euro 2020 debut.
It kicks off on Sunday at 17.00 UK time (16.00 GMT) at the Stadio Olimpico in Rome.
The Azzurri have already qualified for the Round of 16, but are not yet sure of first place in Group A after beating Turkey and Switzerland 3-0.
Wales were held to a 1-1 draw by the Swiss and beat the Turks 2-0, so are in pole position to go through even with a defeat.
Roberto Mancini’s men had a training session at the Coverciano ground this afternoon, ahead of tomorrow’s journey to Rome.
Verratti has not even been on the bench for the first two games, as he ended the PSG season with a knee injury.
He has now fully recovered and could allow Jorginho or Manuel Locatelli a rest against Wales.
Giorgio Chiellini is ruled out by a muscular issue, although the veteran said he stopped in time to avoid a serious problem, with Francesco Acerbi expected to step in.
Alessandro Florenzi and Giovanni Di Lorenzo are competing for the right-back spot, although Rafael Toloi is another candidate.
Leonardo Spinazzola should be rested in favour of Emerson Palmieri, with Andrea Belotti potentially stepping in for Ciro Immobile, who scored in his first two Italy games.
Federico Chiesa is champing at the bit to play after two brief substitute appearances, allowing Lorenzo Insigne some time out.
Domenico Berardi, however, is expected to start the third match in a row.
Italy (4-3-3): G Donnarumma; Toloi, Bonucci, Acerbi, Emerson; Locatelli, Jorginho, Barella; Berardi, Belotti, Chiesa