Fiorentina will look to Lucas Beltran, while Giovanni Simeone is favourite over Giacomo Raspadori in the Napoli attack in the first Supercoppa semi-final tonight.
It kicks off at 19:00 GMT at the Al-Awwal Park in Riyadh, Saudi Arabia.
It is the first time ever that the Supercoppa involves four teams with Serie A and Coppa Italia runners-up meeting the title holders.
Serie A champions Napoli must cope without the injured Alex Meret, Olivera, Natan, Diego Demme plus André Zambo Anguissa and Victor Osimhen on AFCON international duty.
Piotr Zielinski is expected to return to the starting XI after missing the last Serie A game with Salernitana with a slight muscle injury. Simeone is favourite over Raspadori in attack.
Fiorentina have a few players out of action as well: Dodo, Nico Gonzalez and Gaetano Castrovilli are on the treatment table, while Christian Kouamé is on AFCON international duty.
New signing Marco Davide Faraoni could start as a right-back in place of Michael Kayode, while Fabiano Parisi and Cristiano Biraghi will play on the left wing if Josip Brekalo initially goes on the bench.
The winner will face Inter or Lazio in the Final. Nerazzurri and Biancocelesti meet on Friday night in the second semi-final, while the Final will be played on Monday, January 22.
Napoli-Fiorentina, probable line-ups
Napoli (4-3-3): Gollini; Di Lorenzo, Rrahmani, Juan Jesus, Mario Rui; Cajuste, Lobotka, Zielinski; Politano, Simeone, Kvaratskhelia
Fiorentina (4-2-3-1): Terracciano; Faraoni, Milenkovic, Martinez Quarta, Biraghi; Arthur, Duncan; Ikone, Bonaventura, Parisi; Beltran.