Fiorentina seek their first win of the season with Moise Kean and Robin Gosens, while Tijjani Noslin steps in for injured Lazio striker Taty Castellanos.
It kicks off at the Stadio Franchi at 11.30 UK time (10.30 GMT).
The Viola are still looking for their first victory under Raffaele Palladino, after five draws between Serie A and the Conference League, plus last week’s 3-2 loss to Atalanta.
It is finally a full squad at his disposal, as ex-Genoa forward Albert Gudmundsson has shaken off a muscular issue, but is only on the bench and still waiting for his debut.
Former Lazio midfielder Danilo Cataldi faces his old club with David De Gea in goal, while Marin Pongracic is out of action.
The Biancocelesti had a better start under new coach Marco Baroni, picking up two wins, a draw and one defeat.
Castellanos is out with an adductor issue, while Samuel Gigot hurt his shoulder, so Noslin goes into the centre-forward role supported by Boulaye Dia and Mattia Zaccagni.
Gaetano Castrovilli left Fiorentina as a free agent this summer, so he returns to the Stadio Artemio Franchi as an opponent.
Fiorentina vs. Lazio line-ups
Fiorentina: De Gea; Martinez Quarta, Comuzzo, Biraghi; Dodò, Mandragora, Cataldi, Bove, Gosens; Colpani; Kean
Lazio: Provedel; Lazzari, Patric, Gila, Tavares; Guendouzi, Castrovilli; Isaksen, Dia, Zaccagni; Noslin
Player statistic
| Robin Gosens |  |
11' | ||
| 26' | |
Gustav Isaksen | ||
| 41' |  |
Mario Gila (Assist: Nuno Tavares) | ||
| Cristiano Biraghi | |
42' |
| Albert Gudmundsson |  |
49' |
