Venezia are finally back in the Stadio Penzo after restructuring work, but Spezia have a striker shortage.
It kicks off at 14.00 UK time (13.00 GMT).
This is an early relegation dogfight, as the Lagunari were promoted via the play-offs and had lost the opening two games, but got themselves right back on track with a surprise victory away to Empoli.
Luca Lezzerini and Luca Fiordilino are ruled out, with Mattia Aramu, Arnor Sigurdsson and Dor Peretz not fully fit.
As for Spezia, they have struggled badly under new coach Thiago Motta, managing an opening 2-2 draw at Cagliari, then 6-1 loss to Lazio and 1-0 home defeat to Udinese.
Ebrima Colley, Kevin Agudelo and Leo Sena are out of action for at least a month, so Janis Antiste has to adapt as a centre-forward.
Venezia: Maenpaa; Mazzocchi, Caldara, Ceccaroni, Molinaro; Crnigoj, Vacca, Busio; Okereke, Henry, Johnsen
Spezia: Zoet; Amian, Erlic, Nikolaou, Ferrer; S Bastoni, Bourabia, Maggiore; Verde, Antiste, Gyasi