Sassuolo look to Domenico Berardi, Giacomo Raspadori and Gianluca Scamacca against Fiorentina’s Krzysztof Piatek and Jonathan Ikone in a tussle for European ambitions.
It kicks off at the Mapei Stadium in Reggio-Emilia at 19.50 GMT.
You can follow all the build-up and action as it happens and give your views on the LIVEBLOG.
Serie A Wk27 Liveblog: Salernitana-Bologna, Empoli-Juventus, Sassuolo-Fiorentina
These are two of the most exciting teams to watch in Serie A, both under young Italian coaches and with plenty of intriguing talent.
They are also both fresh from giant-killing exploits, as the Neroverdi beat Inter 2-0 at San Siro and the Viola just saw off Atalanta.
Gian Marco Ferrari returns from suspension for Sassuolo, with the usual Azzurri forwards Berardi, Raspadori and Scamacca joined by Hamed Junior Traore.
Nikola Milenkovic sits out a ban for Fiorentina with Lucas Torreira carrying an injury too.
Riccardo Sottil and Piatek again get the nod rather than Riccardo Saponara and Arthur Cabral, but Jonathan Ikone has a rare start instead of Nico Gonzalez, while Bartlomiej Dragowski is confirmed in goal.
Sassuolo: Consigli; Muldur, Chiriches, Ferrari, Kyriakopoulos; Frattesi, Lopez; Berardi, Raspadori, Traore; Scamacca
Fiorentina: Dragowski; Odriozola, Igor, Martinez Quarta, Biraghi; Castrovilli, Amrabat, Maleh; Ikone, Piatek, Sottil