Roma look to Nicolò Zaniolo, Tammy Abraham and Sergio Oliveira against a Genoa side revamped by January signings and new coach Alexander Blessin.
It kicks off at the Stadio Olimpico at 14.00 GMT.
Liveblog: Serie A Week 24 Roma-Genoa, Inter-Milan and Fiorentina-Lazio
The Giallorossi are coming off back-to-back wins over Empoli and Cagliari before the break for international duty.
Leonardo Spinazzola is still out of action, while Lorenzo Pellegrini and Matias Vina are not at 100 per cent.
Zaniolo has been in the news this week amid transfer speculation after director Tiago Pinto confirmed he ‘could not guarantee’ the Italy international would still be at the club next season.
All eyes will therefore be on Zaniolo, who leads the attack with Abraham and Henrikh Mkhitaryan, while Sergio Oliviera pulls the midfield strings with fellow January arrival Ainsley Maitland-Niles on the flank.
Genoa are in penultimate place and run the genuine risk of relegation after a chaotic campaign that saw the club change ownership and three different coaches.
German tactician Blessin made his debut on the bench with a 0-0 draw against Udinese, but Andrea Cambiaso was sent off and is therefore suspended.
Nicolò Rovella and Domenico Criscito are out injured, so Mattia Destro leads the way with support from Caleb Ekuban and Kelvin Yeboah, with Nadiem Amiri on the bench.
Genoa have only one Serie A victory all season, 3-2 over Cagliari on September 12.
Roma: Rui Patricio; Mancini, Smalling, Ibanez; Karsdorp, Cristante, Sergio Oliveira, Maitland-Niles; Mkhitaryan; Zaniolo, Abraham
Genoa: Sirigu; Hefti, Vanheusden, Bani, Vasquez; Sturaro, Badelj, Portanova; Ekuban, Destro, Yeboah