Pedro acts as Lazio False 9 supported by Sergej Milinkovic-Savic, Felipe Anderson and Luis Alberto, while Juventus rely on Alvaro Morata and Federico Chiesa.
It kicks off at the Stadio Olimpico at 17.00 GMT.
This is a head-to-head for fifth place, but also an intriguing battle between Maurizio Sarri and his former club, as Max Allegri returned to the Old Lady two years after his dismissal.
There are major injury problems for both coaches to deal with, as Ciro Immobile is out with a calf injury that also denied him international duty with Italy.
Pedro therefore starts as the False 9 flanked by Felipe Anderson and Mattia Zaccagni, with Luis Alberto and Milinkovic-Savic in midfield.
Adam Marusic has COVID-19, so Manuel Lazzari is the right-back.
Juve are missing Paulo Dybala, Giorgio Chiellini, Mattia De Sciglio, Aaron Ramsey and Federico Bernardeschi.
Moise Kean and Wojciech Szczesny are back in the squad, but after returning from Brazil, Alex Sandro is rested in favour of Luca Pellegrini.
Chiesa is moved into a more advanced role with Morata.
Lazio: Reina; Lazzari, Luiz Felipe, Acerbi, Hysaj; Milinkovic-Savic, Cataldi, Luis Alberto; Felipe Anderson, Pedro, Zaccagni
Juventus: Szczesny; Danilo, Bonucci, De Ligt, Pellegrini; Cuadrado, Locatelli, McKennie, Rabiot; Chiesa, Morata