Juventus need to end their Serie A losing streak, so look to Federico Chiesa, Paulo Dybala and Alvaro Morata after losing Wojciech Szczesny, but Fiorentina have Dusan Vlahovic amid a transfer soap opera.
It kicks off at the Allianz Juventus Stadium in Turin at 17.00 GMT.
Serie A Liveblog: Spezia-Torino, Juventus-Fiorentina, Cagliari-Atalanta
The Bianconeri suffered back-to-back defeats with Sassuolo and Hellas Verona, leaving them 16 points off the top spot.
Although they recovered midweek to qualify for the Champions League Round of 16 with a perfect record, their domestic form has been dismal.
Wojciech Szczesny pulled out with a slight rib injury, so Mattia Perin takes the gloves.
Moise Kean and Mattia De Sciglio are also on the treatment table, so former Fiorentina men Federico Chiesa starts with Morata and Dybala.
Federico Bernardeschi makes way for Adrien Rabiot, while Leonardo Bonucci is not at 100 per cent, so Giorgio Chiellini was named in defence, but the veteran pulled out in the warm-up with an adductor issue, so Daniele Rugani steps in.
Fiorentina are still without Nico Gonzalez, who tested positive for COVID-19 last month, plus injured Bartlomiej Dragowski, Erick Pulgar and Aleksandar Kokorin.
Jose Callejon gets the nod rather than Riccardo Sottil to complete the trident attack with Riccardo Saponara and Juventus transfer target Vlahovic.
All eyes will be on the Serbian striker, who refused to sign a new contract and could be sold in January.
Juventus: Perin; Danilo, De Ligt, Rugani, Alex Sandro; Chiesa, McKennie, Locatelli, Rabiot; Dybala, Morata
Fiorentina: Terracciano; Odriozola, Milenkovic, Martinez Quarta, Biraghi; Bonaventura, Torreira, Duncan; Callejon, Vlahovic, Saponara