Mohamed Fares starts for Genoa after his debut brace secured their first win of the season, while Fiorentina hope Dusan Vlahovic can continue his extraordinary form.
It kicks off at the Stadio Luigi Ferraris, also known as Marassi, at 14.00 UK time (13.00 GMT).
Genoa got off the mark with their first win of the season last week, fighting back from 2-0 down to beat Cagliari 3-2 with a brace from ex-Lazio wing-back Fares on his debut.
He gets his first start here after that remarkable substitute performance, but Felipe Caicedo and Stefano Sturaro are out of action, so Mattia Destro partners Filippo Melegoni upfront.
Fiorentina have had a very strong start to their campaign under new coach Vincenzo Italiano, unlucky in defeat to Roma, then beating Torino and Atalanta 2-1.
Vlahovic has picked up where he left off last season, scoring five goals in four games between Serie A and the Coppa Italia.
Lorenzo Venuti is out after dislocating his shoulder, so Alvaro Odriozola steps in, while Nicolas Gonzalez, Gaetano Castrovilli and Erick Pulgar are in support.
Nikola Milenkovic and Lucas Torreira are only on the bench.
Genoa: Sirigu; Vanheusden, Maksimovic, Criscito; Cambiaso, Toure, Badelj, Rovella, Fares; Melegoni, Destro
Fiorentina: Dragowski; Odriozola, Igor, Martinez-Quarta, Biraghi; Bonaventura, Pulgar, Castrovilli; Callejon, Vlahovic, Gonzalez
Ref: Marinelli