Fiorentina begin life after Dusan Vlahovic by using Arthur Cabral and José Maria Callejon, with Krzysztof Piatek on the bench, while Pedro returns to support Ciro Immobile for Lazio.
It kicks off at the Stadio Artemio Franchi at 19.45 GMT.
Liveblog: Serie A Week 24 Roma-Genoa, Inter-Milan and Fiorentina-Lazio
The Viola sold their top scorer to Juventus in the January transfer window and brought in Arthur Cabral from FC Basel.
The Brazilian striker makes his Fiorentina debut along with Riccardo Sottil and José Maria Callejon.
Alvaro Odriozola sits out a ban and is replaced by Lorenzo Venuti.
Lazio if anything have been weakened in the window, bringing in only winger Jovane Cabral, who is on the bench, while Vedat Muriqi, Denis Vavro, Gonzalo Escalante and Jony were loaned out.
Francesco Acerbi is injured, but Pedro returns after a period on the treatment table and Danilo Cataldi has completed his ban, but starts on the bench.
Fiorentina: Terracciano; Venuti, Milenkovic, Nastasic, Biraghi; Bonaventura, Torreira, Duncan; Sottil, Cabral, Callejòn.
Lazio:Strakosha; Lazzari, Luiz Felipe, Patric, Marusic; Milinkovic-Savic, Lucas Leiva, Luis Alberto; Pedro, Immobile, Zaccagni.