Dries Mertens leads the attack for a Napoli side without Victor Osimhen, while Atalanta look to Luis Muriel in this head-to-head for a top four spot.
It kicks off at the Gewiss Stadium in Bergamo at 14.00 UK time (13.00 GMT).
While La Dea have fallen behind, they do still have a game in hand, but are also the only Italian side remaining in Europe.
The Partenopei are feeling the pressure to keep up with leaders Milan, but must do it without suspended top scorer Osimhen and defender Amir Rrahmani.
Duvan Zapata is on the Atalanta bench for the first time since early February, recovering ahead of schedule from a thigh strain, while Luis Muriel has recovered from an issue with Colombia and starts with support from Ruslan Malinovskyi and Teun Koopmeiners.
Napoli give Mertens a rare start upfront, just days after he became a father to little Ciro Romeo, with Lorenzo Insigne and Matteo Politano.
Giovanni Di Lorenzo, Alex Meret, Adam Ounas and Andrea Petagna are injured, so 21-year-old Alessandro Zanoli gets his first start at right-back.
Fabian Ruiz had a fever this week, so Piotr Zielinski is preferred.
Atalanta: Musso; Scalvini, Palomino, Djimsiti; Hateboer, De Roon, Freuler, Zappacosta; Koopmeiners; Malinovskyi, Muriel
Napoli: Ospina; Zanoli, Juan Jesus, Koulibaly, Mario Rui; Anguissa, Lobotka, Zielinski; Politano, Mertens, Insigne
Same old problem, if you look after line up of both teams only 3 Italian starters on each team, as if we didn’t learn anything from the disastrous World Cup qualifications. How many more disaster must happen that Italy changes and play more Italians in the starting 11