Atalanta have Duvan Zapata back in the starting XI with support from Matteo Pessina, facing Dusan Vlahovic’s Fiorentina.
It kicks off at the Gewiss Stadium in Bergamo at 19.45 UK time (18.45 GMT).
La Dea had a stuttering start to the season, scraping a 2-1 win over Torino and held 0-0 by Bologna.
Luis Muriel is out for several weeks with a muscular tear, but Duvan Zapata does return to the starting XI after his injury issues, supported by Aleksei Miranchuk and Matteo Pessina.
Marten De Roon continues his ban and Hans Hateboer is a long-term absentee.
Fiorentina have been playing exciting and aggressive football under coach Vincenzo Italiano, with Vlahovic continuing the form that saw him attract €70m offers this summer.
Nicolas Gonzalez is rested after returning late from international duty in South America, the same going for Lucas Martinez Quarta and Erick Pulgar.
Bartlomiej Dragowski is back after his ban for a red card against Roma, but Pietro Terracciano still gets the gloves.
Atalanta: Sportiello; Toloi, Palomino, Djimsiti; Maehle, Pasalic, Freuler, Zappacosta; Pessina; Miranchuk, Duvan Zapata
Fiorentina: Terracciano; Venuti, Milenkovic, Igor, Biraghi; Bonaventura, Torreira, Duncan; Callejon, Vlahovic, Sottil