Roma hope to kick-start their season with Paulo Dybala and Tammy Abraham, but Nicolò Zaniolo pulls out, while Fiorentina are ambitious too starting Luka Jovic and Sofyan Amrabat.
It kicks off at the Stadio Olimpico at 19.45 GMT.
Jose Mourinho is back on the touchline after a two-match ban, during which the Giallorossi beat Bologna and came back from 2-0 down in stoppages to draw 2-2 away to Milan.
They also scraped past Genoa 1-0 in the Coppa Italia midweek, a tense match that saw Zaniolo jeered by the home fans, further putting his future at the club in jeopardy.
Eyebrows were therefore raised this afternoon when it was announced Zaniolo was not part of the squad, officially due to a stomach bug.
Lorenzo Pellegrini, who also has a muscular issue to deal with, is therefore pushed into a more advanced role with Dybala and Abraham.
Marash Kumbulla steps in for suspended Roger Ibanez and Georginio Wijnaldum is injured.
Fiorentina have a lot of injury problems going into this game, losing Arthur Cabral for a month to a thigh strain, but also making this trip without Riccardo Saponara, Rolando Mandragora, Lucas Martinez-Quarta and Riccardo Sottil.
Jack Bonaventura can celebrate signing a new contract, starting with Jovic, Christian Kouame and Jonathan Ikone.
Leicester City transfer target Nico Gonzalez is on the bench, as he is still not fully fit after the injury that kept him out of Argentina’s World Cup campaign.
Roma: Rui Patricio; Mancini, Smalling, Kumbulla; Celik, Cristante, Bove, Zalewski; Dybala, Pellegrini; Abraham
Fiorentina: Terracciano; Dodo, Milenkovic, Igor, Biraghi; Amrabat, Duncan; Ikone, Bonaventura, Kouame; Jovic