Napoli do not rotate, so Victor Osimhen, Kvicha Kvaratskhelia and Matteo Politano start, while Atalanta hope to rediscover their form with Rasmus Hojlund and Duvan Zapata.
It kicks off at the Stadio Diego Armando Maradona at 17.00 GMT.
The Partenopei suffered only their second Serie A defeat of the season, falling 1-0 at home to Lazio, yet can extend their lead at the top of the table because Inter were beaten by Spezia last night.
Mario Rui is again suspended after a reaction foul, with Giacomo Raspadori and Hirving Lozano injured, so Politano and Mathias Olivera step in.
Luciano Spalletti does not rotate the squad ahead of Wednesday’s Champions League Round of 16 tie against Eintracht Frankfurt.
Atalanta are still in the race for the top four, despite stuttering recently with one point from the last three rounds.
Teun Koopmeiners joined Hans Hateboer on the treatment table, while Giorgio Scalvini and Davide Zappacosta have recovered.
It had been reported Duvan Zapata would start instead of Rasmus Hojlund, but there is an all-new partnership of these two, sending Ademola Lookman and Jeremie Boga to the bench.
Napoli: Meret; Di Lorenzo, Rrahmani, Kim Min-jae, Olivera; Anguissa, Lobotka, Zielinski; Politano, Osimhen, Kvaratskhelia
Atalanta: Musso; Toloi, Djimsiti, Scalvini; Ruggeri, De Roon, Ederson, Maehle; Pasalic; Zapata, Hojlund
Player statistic
| 16' |  |
Matteo Ruggeri | ||
| Victor Osimhen | |
18' | ||
| 30' | |
Giorgio Scalvini |
| Khvicha Kvaratskhelia (Assist: Victor Osimhen) |
 |
60' | ||
| Amir Rrahmani (Assist: Eljif Elmas) |
|
77' |
Match statistic
