Lazio have the opportunity to climb up to within two points of second place with a victory and Pedro is able to play with a protective mask against desperate Sampdoria.
It kicks off at the Stadio Olimpico at 19.45 GMT.
Sergej Milinkovic-Savic is back in the starting XI after suffering from a stomach bug, which also affected Mattia Zaccagni and Marcos Antonio.
Because of that, Pedro is chosen to complete the trident attack with the aid of a protective mask, having fractured his nose last week against Salernitana.
Alessio Romagnoli and Stefan Radu remain on the treatment table.
They were held to a 0-0 draw by CFR Cluj on Thursday evening, which proved enough to book their place in the Conference League Round of 16.
Sampdoria are still in penultimate place and increasingly desperate to avoid the drop, although they did manage to hold Inter 0-0 before the home defeat to Bologna.
Fabio Quagliarella, Filip Djuricic, Jeison Murillo, Koray Gunter, Ignacio Pussetto and Andrea Conti are out of action.
Sam Lammers leads the way with Manolo Gabbiadini.
Lazio: Provedel; Lazzari, Patric, Casale, Marusic; Milinkovic-Savic, Cataldi, Luis Alberto; Felipe Anderson, Immobile, Pedro
Sampdoria: Audero; Amione, Nuytinck, Zanoli; Leris, Winks, Rincon, Augello; Cuisance; Gabbiadini, Lammers
Player statistic
| 49' |  |
Mehdi Léris | ||
| 67' | |
Manolo Gabbiadini | ||
| Matías Vecino | |
71' | ||
| Nicolò Casale | |
78' | ||
| Luis Alberto |  |
80' |
Match statistic
