Lazio try to build on their momentum for a Champions League place with Felipe Anderson, Sergej Milinkovic-Savic and Luis Alberto, while Nico Gonzalez and Sofyan Amrabat try to revitalise Fiorentina.
It kicks off at the Stadio Olimpico in Rome at 17.00 GMT.
Maurizio Sarri’s men have hit form in 2023 with seven points from three rounds, including last week’s 4-0 hammering of Milan.
Ciro Immobile is still carrying that thigh injury, but Felipe Anderson has filled in admirably as a False 9, here flanked by Mattia Zaccagni and Pedro, with Milinkovic-Savic and Luis Alberto pulling the midfield strings.
Stefan Radu is the only other absentee, with Matias Vecino and Patric not at 100 per cent.
Fiorentina, on the other hand, are coming off back-to-back defeats to Roma and Torino without scoring a single goal.
Dodo was sent off at the Olimpico, so returns to the side here after a one-match ban, while Arthur Cabral, Gaetano Castrovilli, Riccardo Sottil and Lucas Martinez Quarta are injured.
New buy Josip Brekalo only arrived yesterday from VfL Wolfsburg, but Gonzalez gets his first start since the World Cup injury supporting Christian Kouame and Luka Jovic.
Lazio: Provedel; Marusic, Casale, Romagnoli, Hysaj; Milinkovic-Savic, Cataldi, Luis Alberto; Pedro, Felipe Anderson, Zaccagni
Fiorentina: Terracciano; Dodò, Milenkovic, Ranieri, Biraghi; Amrabat, Barak; Kouamè, Bonaventura, Nico Gonzalez; Jovic