Juventus debut Angel Di Maria with Dusan Vlahovic and have Filip Kostic on the bench, while Sassuolo drop Giacomo Raspadori during the on-going transfer negotiations.
It kicks off at the Allianz Juventus Stadium at 19.45 UK time (18.45 GMT).
The Bianconeri ended last season empty-handed for the first time in a decade and the pressure is on Max Allegri to deliver silverware, especially after a very active transfer window.
However, Paul Pogba is out injured for at least six weeks, with Kostic on the bench having only just arrived from Eintracht Frankfurt.
New buys Gleison Bremer and Angel Di Maria are in the starting XI, with Weston McKennie rushed back from a dislocated shoulder to bolster the midfield.
Other absentees are Wojciech Szczesny, Federico Chiesa, Kaio Jorge, suspended Moise Kean and Manchester United-bound Adrien Rabiot – who had to sit out a ban here anyway.
Sassuolo had a conundrum going into this season opener, whether to play Raspadori when he is in advanced negotiations for a move to Napoli, especially as Juventus have also shown interest in the striker.
Maxime Lopez is suspended and Hamed Junior Traore injured, but new signing Andrea Pinamonti is benched too in favour of Gregoire Defrel.
Juventus: Perin; Danilo, Bonucci, Bremer, Alex Sandro; Cuadrado, Zakaria, Locatelli, McKennie; Di Maria, Vlahovic
Sassuolo: Consigli; Muldur, Ayhan, Ferrari, Rogerio; Frattesi, Matheus Henrique, Thorstvedt; Berardi, Defrel, Kyriakopoulos