Juventus unleash the super trident of Dusan Vlahovic, Federico Chiesa and Angel Di Maria against Sofyan Amrabat and Nico Gonzalez’s Fiorentina.
It kicks off at the Allianz Juventus Stadium at 17.00 GMT.
This has always been a bitter rivalry and seeing former Viola stars Vlahovic and Chiesa start against their old club will only crank up the tension further.
Thanks to the 15-point penalty, it is also a head-to-head for position, with the Bianconeri only two points clear of their opponents.
Arkadiusz Milik, Fabio Miretti, Leonardo Bonucci, Juan Cuadrado, Paul Pogba and Kaio Jorge are out of action, so Mattia De Sciglio starts at right-back.
Fiorentina are forced to do without suspended Igor and Rolando Mandragora, while Antonin Barak pulled out with a stomach bug.
Christian Kouame leads the attack flanked by Jonathan Ikone and Nico Gonzalez, leaving both Luka Jovic and Arthur Cabral on the bench.
The Viola are in need of a boost, having managed just one point in the last four Serie A rounds, although they have qualified for the Coppa Italia semi-final against Cremonese.
You can follow the match statistics live here.
Juventus: Szczesny; Danilo, Bremer, Alex Sandro; De Sciglio, Locatelli, Rabiot, Kostic; Chiesa, Di Maria; Vlahovic
Fiorentina: Terracciano; Dodò, Milenkovic, Ranieri, Biraghi; Bonaventura, Amrabat, Duncan; Nico Gonzalez, Kouamé, Ikoné