Fiorentina need a victory to be sure of qualifying for the Conference League ahead of Atalanta and that’s not the only motivation against arch-rivals Juventus, who start Paulo Dybala and Giorgio Chiellini.
It kicks off at the Stadio Artemio Franchi at 19.45 UK time (18.45 GMT).
Serie A Liveblog: Fiorentina-Juventus, Lazio-Verona, Atalanta-Empoli
With Roma and Lazio already sure of the two Europa League spots, there’s only one place in Europe left and that’s the Conference League play-off.
Fiorentina and Atalanta go into the final round level on points, but the Viola have a superior head-to-head record.
A win here would therefore guarantee Vincenzo Italiano’s men finish in seventh place.
Sofian Amrabat returns from suspension, but Youssef Maleh is banned, with Riccardo Sottil and Gaetano Castrovilli injured.
The big surprise is Krzysztof Piatek leading the attack rather than Arthur Cabral.
Juve are missing Danilo, Mattia De Sciglio, Kaio Jorge and Federico Chiesa, though Weston McKennie is back after a long lay-off.
Dybala and Chiellini perhaps thought they’d played their final games in the 2-2 draw with Lazio, but are back in the starting XI.
So is Federico Bernardeschi in his last appearance, as his contract expires on June 30.
Fiorentina need no motivation in this fixture, as Juve are their arch-rivals, a tension further exacerbated when Dusan Vlahovic moved in January and he is benched this evening.
Fiorentina: Terracciano; Venuti, Milenkovic, Igor, Biraghi; Bonaventura, Amrabat, Duncan; Nico Gonzalez, Piatek, Saponara
Juventus: Perin; De Ligt, Bonucci, Chiellini; Bernardeschi, Locatelli, Miretti, Rabiot, Alex Sandro; Dybala, Kean