Juventus rest Dusan Vlahovic, but start Leandro Paredes, Angel Di Maria and Arkadiusz Milik for the difficult trip to Fiorentina, where Luka Jovic is eager to end their goal drought.
It kicks off at the Stadio Artemio Franchi at 14.00 UK time (13.00 GMT).
The Bianconeri got back on track after two consecutive draws with a 2-0 win over Spezia, though it was again far from a vintage performance.
Vlahovic scored with another sensational free kick, but he is rested ahead of the Champions League clash with Paris Saint-Germain.
Instead, Milik gets his first start, as does new signing and ex-PSG midfielder Paredes, thrown straight into the fray.
Wojciech Szczesny, Adrien Rabiot, Paul Pogba, Federico Chiesa and Kaio Jorge are injured, with Leonardo Bonucci carrying muscular issues.
Fiorentina have failed to score in their last four matches between Serie A and the Conference League play-offs, so need to break that drought.
Giacomo Bonaventura is on compassionate leave following the death of his father, while Alfred Duncan and Gaetano Castrovilli are injured.
Jovic leads the attack with Christian Kouame and Riccardo Sottil, as Nico Gonzalez still has an ankle problem.
Fiorentina: Terracciano; Dodo, Milenkovic, Igor, Biraghi; Maleh, Amrabat, Barak; Kouame, Jovic, Sottil
Juventus: Perin; Cuadrado, Danilo, Bremer, Alex Sandro; Locatelli, Paredes, McKennie; Di Maria, Milik, Kostic