Fiorentina keep squad rotation to an absolute minimum against an Atalanta side with Rasmus Hojlund and Duvan Zapata.
It kicks off at the Stadio Artemio Franchi at 19.45 UK time (18.45 GMT).
You can follow all the build-up and action as it happens on the LIVEBLOG.
The Viola are one of the form teams in Europe at the moment, putting together 11 victories and two draws in all competition since last tasting defeat in mid-February.
That included away victories in the first leg of the Conference League quarter-final and Coppa Italia semi-final.
Sofyan Amrabat is out with a muscular issue and Salvatore Sirigu’s season is over, while Arthur Cabral and Nico Gonzalez get no rest.
Atalanta are still in the fight for European places, despite a recent slide of just two wins in the last seven rounds.
Ademola Lookman was a late addition to the absentee list with Matteo Ruggeri and Hans Hateboer, while Teun Koopmeiners returns to the starting XI and Mario Pasalic is only fit for the bench.
Marco Sportiello faces his former club rather than Juan Musso in goal.
Fiorentina: Terracciano; Dodo, Milenkovic, Martinez Quarta, Terzic; Castrovilli, Mandragora, Barak; Ikone, Cabral, Gonzalez
Atalanta: Sportiello; Toloi, Djimsiti, Scalvini; Zappacosta, De Roon, Koopmeiners, Maehle; Ederson; Hojlund, Duvan Zapata
Player statistic
37' | Joakim Maehle | |||
38' | Joakim Maehle |
Arthur Cabral | 56' | |||
60' | Éderson | |||
71' | Rafael Tolói | |||
Lucas Martínez Quarta | 75' |