Federico Chiesa is favourite over Kenan Yildiz in the Juventus attack, while Fiorentina will look to Andrea Belotti and Nico Gonzalez.
It kicks off at 19:45 UK time (GMT+1) at the Allianz Stadium in Turin.
The Bianconeri are desperate for a win having earned just seven points in the last nine Serie A games. Massimiliano Allegri will release his squad list this morning, but Arkadiusz Milik is expected to be the only Juventus player out of action through injury, while Paul Pogba and Nicolò Fagioli are suspended.
Chiesa had a fever this week, but only skipped a training session so he is favourite to start alongside Dusan Vlahovic.
Their former club Fiorentina can rely on a full squad and coach Vincenzo Italiano will line up the best team despite Thursday’s Conference League quarter-finals first leg against Viktoria Plzen.
Cristiano Biraghi is expected to return to the starting XI alongside Juventus loanee Arthur. Gonzalez, Lucas Beltran and Riccardo Sottil will play behind Belotti.
Juventus vs. Fiorentina – probable line-ups
Juventus (3-5-2): Szczesny; Gatti, Bremer, Danilo; Cambiaso, McKennie, Locatelli, Rabiot, Kostic; Chiesa, Vlahovic.
Fiorentina (4-2-3-1): Terracciano; Dodo, Milenkovic, Ranieri, Biraghi; Arthur, Duncan; Nico Gonzalez, Beltran, Sottil; Belotti.