Fiorentina will look to Andrea Belotti and Nico Gonzalez against Napoli who are expected to rely on Giacomo Raspadori to replace the injured Victor Osimhen.
It kicks off at 20:45 CET at the Stadio Franchi in Florence.
The Viola have qualified for the Conference League Final and still hope to earn a spot in the Europa League for 2024-25. Vincenzo Italiano won’t rotate much in what could be his final home game at the club.
Belotti, Giacomo Bonaventura and Nico Gonzalez are all expected to start and only Riccardo Sottil is unavailable for the Tuscans.
Napoli, on the other hand, must cope without Piotr Zielinski, Pierluigi Gollini, Mario Rui and Osimhen.
Italy international Raspadori is favourite to start in attack alongside Matteo Politano and Khvicha Kvaratskhelia, but ex-Fiorentina striker Simeone also has a chance to start up front.
Napoli currently sit 9th in the Serie A table and a Conference League spot is realistically the best result they can achieve in the final two games of the season.
Fiorentina vs Napoli – probable line-ups
Fiorentina (4-2-3-1): Terracciano; Dodô, Milenkovic, Martínez Quarta, Biraghi; Arthur, Bonaventura; Nico Gonzalez, Beltran, Kouamé; Belotti.
Napoli (4-3-3): Meret; Di Lorenzo, Rrahmani, Juan Jesus, Olivera; Anguissa, Lobotka, Cajuste; Politano, Raspadori, Kvaratskhelia.