Vincenzo Italiano is likely to rotate the team that started against Cremonese in the Coppa Italia semi-final when Sampdoria visit the Stadio Artemio Franchi on Sunday evening.
Lucas Martinez Quarta is expected to return to the back line in place of Nikola Milenkovic, although the rest of the defensive structure is likely to remain the same.
Wholesale changes in midfield could be on the cards with Sofyan Amrabat and Gaetano Castrovilli predicted to return to the pack.
Christian Kouame, Antonin Barak and Andrea Sottil are expected to provide support for Luka Jovic in attack, who could come in to replace Arthur Cabral.
Kick off in Tuscany is at 17.00 UK time.
Fiorentina (4-2-3-1): Terracciano; Dodo, Quarta, Igor, Terzic; Amrabat, Castrovilli; Kouame, Barak, Sottil; Jovic.
Samp (3-4-2-1): Ravaglia; Murillo, Gunter, Amione; Zanoli, Winks, Rincon, Augello; Leris, Cuisance; Gabbiadini.
Thiago Motta is expected to name an unchanged back four at the Dall’Ara when Juventus come to town. Nicolas Dominiguez and Jerdy Schouten are likely to retain their places in midfield as well.
Nikola Moro could return to the starting XI to line up alongside Lewis Ferguson and Riccardo Orsolini behind Musa Barrow in attack.
Massimiliano Allegri is also likely to chop and change the Juventus team that lined up against Inter in the Coppa Italia semi-final in the midweek round of fixtures.
Juan Cuadrado is tipped to start at full-back, with Gatti and Danilo predicted to start in a Bianconeri back four. Alex Sandro will take his place at left-back.
In midfield, Nicolo Fagioli is expected to take the place of Fabio Miretti in the starting line-up, whilst Angel Di Maria is likely to miss out.
In attack, we could see a front three of Federico Chiesa, Arkadiusz Milik and Filip Kostic.
Kick off comes at 19.45 UK time.
Bologna (4-3-3-1): Skorupski; Posch, Soumaoro, Lucumi, Kyriakopoulos; Dominguez, Schouten; Moro, Ferguson, Orsolini; Barrow.
Juventus (4-3-3): Szczesny; Cuadrado, Gatti, Danilo, Alex Sandro; Fagioli, Locatelli, Rabiot; Chiesa, Milik, Kostic.