Atalanta are expected to start with Ruslan Malinovskyi next to Duvan Zapata against Villarreal in the Champions League.
The match kicks off at 20.00 UK time.
The Orobici are back in the Champions League and fly to Spain to visit Villarreal, who arrive after winning the Europa League last term.
The Nerazzurri are aiming to bounce back from a defeat against Fiorentina in Serie A, as they prepare to kick-off their third consecutive season in the Champions League.
Young Boys and Manchester United complete the group, but it’s Unai Emery’s men who await in the first leg, who are expected to play in a classic 4-4-2 formation.
Boulaye Dia and Gerard Moreno seem set to start up front, with Yeremi Pino and Alberto Moreno on the sides.
Gian Piero Gasperini is expected to rely on Zapata in attack, as Luis Muriel is unavailable due to injury.
The Colombian will spearhead the attack, with Malinovskyi by his side and Matteo Pessina behind as an attacking midfielder.
De Roon and Remo Freuler should start in the middle of the park, with Robin Gosens returning at left. On the right, Davide Zappacosta and Joakim Maehle fight for the starting position.
Rafael Toloi, Jose Palomino and Berat Djimsiti are expected to start in front of Juan Musso, who returns in goal after missing the Fiorentina game.
Villarreal (probable): Rulli; Foyth, Raul Albiol, Mandi, Estupinan; Pino, Trigueros, Capoue, A. Moreno; G. Moreno, Dia
Atalanta (probable): Musso; Toloi, Palomino, Djimsiti; Zappacosta, De Roon, Freuler, Gosens; Pessina; Malinovskyi, Zapata
Hopefully they get a result. A defeat here leaves them with plenty to do to qualify.
Yeah, this is the big game really. Should be a cracker!