Luciano Spalletti will look to Victor Osimhen and Lorenzo Insigne, while Sinisa Mihajlovic must cope without Roberto Soriano, Adama Soumaoro and Marko Arnautovic.
It kicks off at 19.45 UK time at the Stadio Maradona.
The Partenopei need a win to catch Milan on top of the Serie A table and move seven points clear of Inter in third.
Luciano Spalletti is not expected to rotate his squad much as Osimhen and Insigne are expected to start up front. Kostas Manolas, Kevin Malcuit and Adam Ounas are injured.
Bologna‘s Soumaoro and Soriano saw red against Milan over the weekend, so they are serving a one-match ban.
Marko Arnautovic is not fully fit and was not included in the Rossoblu squad list for the trip to the Stadio Maradona.
Napoli: Ospina; Di Lorenzo, Rrahmani, Koulibaly, Mario Rui; Anguissa, Fabian Ruiz, Zielinski; Lozano, Osimhen, Insigne.
Bologna: Skorupski; De Silvestri, Medel, Theate; Skov Olsen, Dominguez, Schouten, Svanberg, Hickey; Barrow, Orsolini.
