Giorgio Chiellini is back in training, but unlikely to be risked against Austria, while Italy must choose between Manuel Locatelli and Marco Verratti.
The Azzurri will play Austria at Wembley Stadium in London on Saturday in the Euro 2020 Round of 16.
Roberto Mancini’s men won all three Group A games, beating Turkey and Switzerland 3-0, then Wales 1-0 using a second-string team.
Chiellini and Alessandro Florenzi were out injured for the Wales match, and Florenzi has still not resumed training.
The Juventus defender was spotted running in today’s session at the Coverciano ground, but it remains to be seen whether he’ll be risked from the start this weekend.
It is likely Alessandro Bastoni or Francesco Acerbi will be used instead with Leonardo Bonucci, while Giovanni Di Lorenzo or Rafael Toloi will be the right-back.
The other major doubt remains in midfield, as Verratti has now recovered from a knee issue sustained with PSG and played well in the 1-0 win over Wales.
Locatelli had been a revelation in the first two matches, so Mancini has to pick between them for the next game.
Nicolò Barella and Jorginho are confident of their places in the three-man midfield, while Domenico Berardi, Ciro Immobile and Lorenzo Insigne are the first choice front three, despite the fact Federico Chiesa was named Man of the Match against Wales.
Italy (probable): Donnarumma; Di Lorenzo, Acerbi, Bonucci, Spinazzola; Barella, Jorginho, Verratti/Locatelli; Berardi, Immobile, Insigne