Fiorentina need a positive result in London to continue their Europa League campaign and visit injury-hit Tottenham.
It kicks off at 20.05 GMT, follow all the action as it happens from this game, Lazio-Galatasaray and Napoli-Villarreal on our LIVEBLOG.
The first leg ended 1-1 at the Stadio Artemio Franchi, meaning the Viola need to score at least one goal tonight to have any chance of progressing to the Round of 16.
Fiorentina need a positive result in London to continue their Europa League campaign and visit injury-hit Tottenham.
It kicks off at 20.05 GMT, follow all the action as it happens from this game, Lazio-Galatasaray and Napoli-Villarreal on our LIVEBLOG.
The first leg ended 1-1 at the Stadio Artemio Franchi, meaning the Viola need to score at least one goal tonight to have any chance of progressing to the Round of 16.
Both these sides have some huge domestic games coming up, as on Monday night third-placed Fiorentina take on Napoli in second.
Borja Valero starts despite struggling with flu this week, while Federico Bernardeschi, Josip Ilicic and Nikola Kalinic complete the attack.
Milan Badelj gets the nod in midfield on his 27th birthday and Facundo Roncaglia is back from his ban, albeit on the bench.
Tottenham have to do without injured Harry Kane, Mousa Dembele, Tom Carroll, Jan Vertonghen and Clinton Njie, but goalkeeper Hugo Lloris and ex-Roma talent Erik Lamela are back in the line-up.
Fiorentina knocked Spurs out of the Europa League at this stage last season, but the 1-1 draw was in London and they won 2-0 in Florence.
Spurs have never lost a European home game against Italian opposition, earning three wins and three draws. They are also unbeaten in their last eight continental home fixtures, including six victories.
The Viola did get two victories, one draw and two losses in England during UEFA competition.
Sousa’s men have oddly been stronger in their away fixtures in Europe this season, registering two victories and a 2-2 result at Basel.
Tottenham: Lloris; Trippier, Alderweireld, Wimmer, Davies; Mason, Dier; Lamela, Alli, Eriksen; Chadli
Tottenham bench: Vorm, Rose, Carter-Vickers, Bentaleb, Onomah, Winks, Son
Fiorentina: Tatarusanu; Tomovic, Gonzalo Rodriguez, Astori, Alonso; Vecino, Badelj, Borja Valero; Bernardeschi, Kalinic, Ilicic
Fiorentina bench: Lezzerini, Satalino, Pasqual, Fernandez, Blaszczykowski, Zarate, Babacar