Roma need only a point against Real Madrid to be sure of a place in the Champions League Round of 16, but Edin Dzeko makes way for Patrik Schick.
It kicks off at the Stadio Olimpico at 20.00 GMT.
You can follow the build-up and action as it happens from that game and Juventus v Valencia on the LIVEBLOG.
The Giallorossi lost their opening fixture 3-0 in Madrid on September 19, but a lot has changed since then.
Roma need only a point against Real Madrid to be sure of a place in the Champions League Round of 16, but Edin Dzeko makes way for Patrik Schick.
It kicks off at the Stadio Olimpico at 20.00 GMT.
The Giallorossi lost their opening fixture 3-0 in Madrid on September 19, but a lot has changed since then.
Roma won the next three games, while Real lost away to CSKA Moscow and sacked Coach Julen Lopetegui, replacing him with former player Santiago Solari.
It had been an ideal start to his tenure up until Saturday, when suffering a shock 3-0 defeat at the hands of Liga minnows Eibar.
The two clubs go into this game as joint group leaders on nine points each.
The Merengues have some injury problems, missing key midfielder Casemiro and goalkeeper Keylor Navas.
Also absent are Nacho, Alvaro Odriozola and Sergio Regulion, all of them defenders.
Isco had been expected to step in amid transfer rumours, with Gareth Bale and Lucas Vazquez supporting Karim Benzema.
However, in a shock move, Isco is not even chosen for a spot on the bench. He hasn’t started any of Solari’s five games in charge and is now seemingly pushed out completely.
Roma have similar concerns, as they returned from the break for international duty with an embarrassing defeat at Udinese and fans turned on the club for not investing enough.
Coach Eusebio Di Francesco is forced to do without injured Daniele De Rossi, Lorenzo Pellegrini, Javier Pastore and Diego Perotti.
Edin Dzeko and goalkeeper Robin Olsen are carrying muscular issues, with the striker replaced by Patrik Schick.
Teenager Nicolò Zaniolo was given his surprise Champions League debut at the Bernabeu and he again plays here Cengiz Under and Stephan El Shaarawy.
These sides have met 11 times in the Champions League and the Blancos have won seven, drawing once and losing three.
The victories were in 2002, then both home and away in 2007-08.
Roma will be glad to see referee Clement Turpin, as he was also officiating in last season’s quarter-final when they beat Barcelona 3-0 here to overturn a 4-1 first leg defeat.
Roma: Olsen; Florenzi, Manolas, Fazio, Kolarov; Nzonzi, Cristante; Under, Zaniolo, El Shaarawy; Schick
Roma bench: Mirante, Karsdorp, Jesus, Marcano, Coric, Santon, Kluivert
Real Madrid: Courtois; Carvajal, Varane, Ramos, Marcelo; Modric, Kroos, M Llorente; Bale, Benzema, Lucas Vazquez
Real Madrid bench: Casilla, Mariano, Valverde, Asensio, Dani Ceballos, Vinicius, Javi Sanchez
Ref: Turpin (FRA)