Lazio and Fiorentina go head-to-head for European football, but both sides are forced to make changes at the Stadio Olimpico.
It kicks off at 19.45 GMT, follow all the build-up and action to this and Genoa-Palermo on the Liveblog.
The Aquile have the chance to leapfrog Milan and go into joint third place with Napoli, just one point off their bitter local rivals Roma.
Lazio and Fiorentina go head-to-head for European football, but both sides are forced to make changes at the Stadio Olimpico.
It kicks off at 19.45 GMT, follow all the build-up and action to this and Genoa-Palermo on the Liveblog.
The Aquile have the chance to leapfrog Milan and go into joint third place with Napoli, just one point off their bitter local rivals Roma.
Their only defeat in the last 11 rounds has been in the Derby della Capitale, so Simone Inzaghi’s men got right back on track with a 2-1 victory away to Sampdoria.
This is the first of two huge games to close out 2016, as Lazio travel to Inter on Wednesday night.
Marco Parolo is suspended, so Danilo Cataldi steps in to support Felipe Anderson, Ciro Immobile and Keita Balde Diao.
Federico Marchetti returns in goal following a muscular injury.
Fiorentina beat Palermo and Sassuolo by 2-1 results, but they were far from convincing and lost 1-0 at Genoa in Thursday’s rescheduled Week 3 tie.
Paulo Sousa is under pressure from fans who want to see him sacked, but also makes the trip without suspended Carlos Salcedo and Milan Badelj.
Borja Valero is a big loss in midfield due to injury, so there’s more riding on Nikola Kalinic, Federico Bernardeschi and Josip Ilicic.
Gonzalo Rodriguez is ruled out by gastroenteritis, so Sebastien De Maio steps in.
These sides haven’t shared the spoils since a goalless Olimpico draw in October 2013, followed by four consecutive Lazio victories and last season’s 4-2 Fiorentina win here.
Lazio: Marchetti; Bastos, De Vrij, Radu; Felipe Anderson, Cataldi, Biglia, Milinkovic-Savic, Lulic; Immobile, Keita
Lazio bench: Strakosia, Vargic, Hoedt, Patric, Lukaku, Kishna, Basta, Wallace, Romero, Lombardi, Murgia, Rossi
Fiorentina: Tatarusanu; Tomovic, De Maio, Astori, Olivera; Sanchez, Vecino; Tello, Ilicic, Bernardeschi; Kalinic
Fiorentina bench: Lezzerini, Dragoswki, Diks, Cristoforo, Chiesa, Milic, Zarate, Perez, Maistro, Babacar