Fiorentina are forced to make changes as they welcome a Sassuolo side again decimated by injuries.
It kicks off at 18.00 GMT, follow the build-up and action on the Liveblog.
The Viola are yet to manage two consecutive Serie A victories this season and are fresh from a Europa League victory over Qarabag in Azerbaijan on Thursday evening.
Fiorentina are forced to make changes as they welcome a Sassuolo side again decimated by injuries.
It kicks off at 18.00 GMT, follow the build-up and action on the Liveblog.
The Viola are yet to manage two consecutive Serie A victories this season and are fresh from a Europa League victory over Qarabag in Azerbaijan on Thursday evening.
They’ve been paired with Borussia Monchengladbach for the Round of 32, but Sassuolo are out of the continental competition after an injury crisis took its toll.
The Neroverdi had started very strong with two early wins after sailing through the preliminary round, but losing Domenico Berardi in August has been a devastating blow.
He’s joined on the treatment table by Simone Missiroli, Emanuele Terranova, Alfred Duncan, Matteo Politano, Timo Letschert and Davide Biondini.
Francesco Acerbi also sustained a knock in Friday’s Europa League defeat to Genk, a game that was rescheduled from Thursday evening due to fog.
Gregoire Defrel leads the trident with support from Federico Ricci and Antonino Ragusa.
Fiorentina have Nenad Tomovic suspended and Carlos Toledo injured, but Nikola Kalinic returns to the attack and is hoping for his first Serie A home goal since April against Juventus.
Federico Bernardeschi and Josip Ilicic start, but Borja Valero has not yet shaken off a knock picked up in Azerbaijan and is not even on the bench.
Khouma Babacar isn't on the bench either, as he caught a cold in Baku and is running a temperature.
These sides have met six times in Serie A, home and away, with a lone 4-3 Sassuolo victory, two draws and three Fiorentina wins.
They also crossed paths in Serie C2 after Fiorentina’s bankruptcy and it was a 0-0 with Sassuolo at the Stadio Franchi that sealed their promotion in 2004.
Fiorentina: Tatarusanu; Salcedo, Gonzalo Rodriguez, Astori, Maxi Olivera; Badelj, Vecino; Chiesa, Ilicic, Bernardeschi; Kalinic
Fiorentina bench: Lezzerini, Dragowski, De Maio, Milic, Cristoforo, Hagi, Zarate, Sanchez, Tello, Perez
Sassuolo: Consigli; Gazzola, Cannavaro, Acerbi, Peluso; Pellegrini, Magnanelli, Sensi; Ricci, Defrel, Ragusa
Sassuolo bench: Pomini, Pegolo, Antei, Iemmello, Matri, Pierini, Lirola, Mazzitelli, Dell’Orco, Adjapong