There is a very tense atmosphere at the Stadio Olimpico, where Lazio fans are jeering their own players ahead of the match with Salernitana.
It kicks off at 19.45 UK time (18.45 GMT) to open the Serie A Week 32 fixtures.
The Aquile are coming off back-to-back defeats against Juventus in the Coppa Italia semi-final first leg and bitter rivals Roma in the Derby della Capitale in Serie A.
Lazio fans made their feelings heard when the squad ran out for the warm-up, loudly jeering the players throughout the preparation.
The ultras in the Curva Nord also aimed insulting chants and banners at the squad, who they blame for a poor campaign, especially compared to securing second place last season.
A banner in the Curva Nord read: ‘You are not worthy to wear OUR shirt.’
Maurizio Sarri resigned on March 13 and Igor Tudor took over a week later, overseeing a 1-0 win over Juventus in Serie A, then those two losses.
Lazio are currently eighth in the Serie A table, nine points adrift of their rivals Roma and fifth place.