Paul Pogba, Federico Chiesa and Fabio Miretti are available for Juventus, but Mattia De Sciglio misses out, while Lorenzo Pellegrini hopes to recover in time for Roma.
It kicks off at the Stadio Olimpico on Sunday at 19.45 GMT.
Max Allegri released his squad list making the journey to the Eternal City, as Manuel Locatelli returns from suspension, while Miretti is back after an injury.
Pogba and Chiesa are both expected to start on the bench after making their comebacks as substitutes in the Derby della Mole on Tuesday night.
De Sciglio has a muscular issue, joining Arkadiusz Milik and Kaio Jorge on the treatment table.
Roma have Chris Smalling back from his ban and Tammy Abraham should get his first start since that nasty cut just under his eye, able to play without the protective mask.
The big question mark is around captain Pellegrini, who is suffering from flu symptoms, while Ola Solbakken has a muscular twinge and is a major doubt.