Gianluca Scamacca gets his debut Italy call-up, while Nicolò Zaniolo returns after injury for the 2022 World Cup qualifiers against Bulgaria, Switzerland and Lithuania.
Coach Roberto Mancini has called up 34 players for the three matches, the first since the Nazionale triumphed at Wembley in UEFA EURO 2020.
The Azzurri are unbeaten in 34 competitive games, just 90 minutes off the all-time record held by Spain, set from 2007 to 2009.
Italy are top of their World Cup qualifying group C after winning all three fixtures in March.
Of the 26 players at EURO 2020, 25 are present in the squad that will start work on Monday, save for injured Leonardo Spinazzola.
Sassuolo-owned striker Scamacca gets his first senior call-up, while Roma man Zaniolo returns after his injury nightmare of two torn anterior cruciate ligaments in one year.
Italy will play Bulgaria on September 2 at the Stadio Artemio Franchi in Florence.
They’ll then visit Switzerland at the St Jakob Park in Basel on Sunday September 5, before hosting Lithuania on Wednesday September 8 at the Mapei Stadium in Reggio-Emilia.
GK: Gianluigi Donnarumma (Paris Saint Germain), Pierluigi Gollini (Tottenham), Alex Meret (Napoli), Salvatore Sirigu (Genoa);
D: Francesco Acerbi (Lazio), Alessandro Bastoni (Inter), Cristiano Biraghi (Fiorentina), Leonardo Bonucci (Juventus), Giorgio Chiellini (Juventus), Giovanni Di Lorenzo (Napoli), Emerson Palmieri (Lyon), Alessandro Florenzi (Milan), Manuel Lazzari (Lazio), Gianluca Mancini (Roma), Rafael Toloi (Atalanta);
M: Nicolò Barella (Inter), Gaetano Castrovilli (Fiorentina), Bryan Cristante (Roma), Frello Jorge Luiz Jorginho (Chelsea), Manuel Locatelli (Juventus), Lorenzo Pellegrini (Roma), Matteo Pessina (Atalanta), Stefano Sensi (Inter), Marco Verratti (Paris Saint Germain), Nicolò Zaniolo (Roma);
F: Andrea Belotti (Torino), Domenico Berardi (Sassuolo), Federico Bernardeschi (Juventus), Federico Chiesa (Juventus), Ciro Immobile (Lazio), Lorenzo Insigne (Napoli), Moise Kean (Everton), Giacomo Raspadori (Sassuolo), Gianluca Scamacca (Sassuolo)