Gennaro Gattuso makes his Italy debut with Moise Kean and Mateo Retegui leading the attack together against Estonia in what seems to be a 4-2-3-1 formation.
It kicks off at the Stadio di Bergamo at 19.45 UK time (18.45 GMT).
You can follow all the build-up and action as it happens on the Liveblog.
Luciano Spalletti was sacked after the opening two games of this 2026 World Cup qualifying campaign, a 3-0 defeat in Norway and 2-0 victory over Moldova in June.
Italy vs Estonia – where to watch on TV
The game is available to watch on Amazon Prime and the Prime Video app in the UK. Fans in the USA can watch the game on Fubo and ViX, while DAZN will broadcast Italy-Estonia in Canada.
Gianluca Scamacca was forced to withdraw with a knee injury, so Kean and Retegui both start upfront, along with Mattia Zaccagni and Matteo Politano in a change to the 4-2-3-1 formation.
Nicolò Barella and Sandro Tonali form the midfield, with Riccardo Calafiori and Alessandro Bastoni in central defence.
Gianluigi Donnarumma gets his first game since joining Manchester City from Paris Saint-Germain.
Marco Carnesecchi, Giovanni Leoni, Giovanni Fabbian and Daniel Maldini are sent to the stands.
Estonia have already played four matches in this group, losing 2-1 to Israel when down to 10 men, winning 3-2 away to Moldova, then suffering a 3-1 home defeat to Israel and just 1-0 to Norway.
Their star man is goalkeeper Karl Hein, who plays for Werder Bremen and is also the captain at age 23.
Italy vs. Estonia official line-ups
Italy: Donnarumma; Di Lorenzo, Calafiori, Bastoni, Dimarco; Barella, Tonali; Politano, Kean, Zaccagni; Retegui
Italy bench: Meret, Vicario, Bellanova, Gatti, Locatelli, Rovella, Raspadori, Pio Esposito, Frattesi, Orsolini, Cambiaso, Mancini
Estonia: Hein; Schjonning-Larsen, Paskotsi, Kuusk, Saliste; Palumets, Shein; Kristal, Kait, Sinyaviskiy; Sappinen
Estonia bench: Igonen, Vallner, Peetson, Saarma, Anier, Yakovlev, Ainsalu, J Tamm, Miller, Tammik, Tur, Soomets
Ref: Joao Pinheiro (POR)
Player statistic
| 31' |  |
Patrik Kristal |
| Moise Kean (Assist: Mateo Retegui) |
 |
58' | ||
| Alessandro Bastoni | |
64' | ||
| Mateo Retegui (Assist: Giacomo Raspadori) |
|
69' | ||
| Giacomo Raspadori (Assist: Matteo Politano) |
|
71' | ||
| 77' | |
Maksim Paskotsi | ||
| Mateo Retegui (Assist: Andrea Cambiaso) |
|
89' | ||
| Alessandro Bastoni (Assist: Giacomo Raspadori) |
|
90+2' | ||
| 90+5' | |
Henri Anier |
Match statistic
Di Lorenzo again. P*rca miseria.