Fiorentina named their squad for the Europa Conference League play-off against FC Twente, missing injured Igor and Szymon Zurkowski, with Luka Jovic expected to start.
The first leg kicks off at the Stadio Artemio Franchi on Thursday at 20.00 UK time (19.00 GMT).
The place in the group phase will be confirmed next Thursday in the Netherlands.
Coach Vincenzo Italiano has to do without injured defender Igor and midfielder Zurkowski.
They have been replaced for this fixture by Marco Benassi and Alessandro Bianco.
Meanwhile, Aleksandar Kokorin has been excluded, as Fiorentina are trying to find a new club for him this summer.
According to Sky Sport Italia, there should be some changes from the XI that beat Cremonese 3-2 in Serie A at the weekend.
Pietro Terracciano could be used in goal, with Dodo and Rolando Mandragora getting their first starts for the Viola.
Jovic and Nico Gonzalez should again lead the trident attack, but with Riccardo Sottil completing the trio.
Fiorentina (probable): Terracciano; Dodo, Milenkovic, Martinez Quarta, Biraghi; Bonaventura, Amrabat, Mandragora; Nico Gonzalez, Jovic, Sottil
Fiorentina squad for FC Twente:
Amrabat, Benassi, Bianco, Biraghi, Bonaventura, Cerofolini, Dodo, Duncan, Gollini, Gonzalez, Ikone, Jovic, Kouame, Maleh, Mandragora, Martinez Quarta, Cabral, Milenkovic, Nastasic, Ranieri, Saponara, Sottil, Terracciano, Terzic, Venuti